Medicinal Biochemistry practicals | Pharm.D. Year 1 Practicals
Teaching material only. These details are not real. These are only for teaching purposes and to make things understandable. Assumed observations and fictional cases are teaching examples, not measured results, clinical decisions, or dosing advice.
This subject contains 21 practical entries in the Pharmacy Council of India's Pharm.D. Regulations, 2008. All 21 source articles and labelled static diagrams are stored in this folder. Illustrative calculations explicitly say pre-assumed values. These are teaching notes for review by Moose before publication.
Topic map
| PCI item range | Topic | Articles |
|---|---|---|
| 1 to 7 | Urine constituents and quantitative urine or serum assays | 7 |
| 8 to 14 | Folin-Wu filtrate, blood analytes, enzymes, urea, and serum protein | 7 |
| 15 to 19 | Bilirubin, glucose oxidase, amylase, and buffers | 5 |
| 20 to 21 | Lipid profile and serum sodium, calcium, potassium | 2 |
Begin with the practicals index and Serum Sodium, Calcium, and Potassium.
Review note
The East Point teaching manual does not provide dedicated methods for the final two PCI entries. No patient-specific diagnosis or treatment is supplied.
Sources
- Pharmacy Council of India. Pharm.D. Regulations, 2008.
- East Point College of Pharmacy. Medicinal Biochemistry Practical Manual.
Practical exercises
1. Qualitative Analysis of Normal Constituents of Urine
To identify selected constituents expected in urine by recording the observations of approved qualitative tests. The PCI syllabus lists this as the first Medici
2. Qualitative Analysis of Abnormal Constituents of Urine
To screen a teaching urine specimen for selected abnormal constituents and document the limits of each qualitative result. The PCI syllabus lists this as the se
3. Quantitative Estimation of Urine Sugar by Benedict's Reagent Method
To describe and calculate a source-based Benedict quantitative titration of urine reducing sugar. This historical method is listed in the PCI Medicinal Biochemi
4. Quantitative Estimation of Urine Chloride by Volhard's Method
To explain a Volhard back titration for urine chloride and calculate the specimen fraction represented by the manual's filtrate aliquot. A chloride concentratio
5. Quantitative Estimation of Urine Creatinine by Jaffe's Method
To calculate urine creatinine from a blank-corrected colorimetric comparison with a verified standard. This is the fifth Medicinal Biochemistry practical in the
6. Quantitative Estimation of Urine Calcium by Precipitation
To explain precipitation of urine calcium as calcium oxalate and calculate a source-based calcium factor for a subsequent permanganate titration. This is the si
7. Quantitative Estimation of Serum Cholesterol by Liebermann-Burchard's Method
To examine the principle of the historical Liebermann-Burchard color reaction and audit the standard calculation before a serum cholesterol value is reported. P
8. Preparation of Folin-Wu Filtrate from Blood
To explain how a deproteinized blood filtrate is obtained for historical Folin-Wu colorimetric work and record the calculation and quality checks for a source-b
9. Quantitative Estimation of Blood Creatinine
To analyze the source method for blood creatinine, calculate the blank-corrected standard-response ratio, and identify the specimen-matrix discrepancy in the co
10. Quantitative Estimation of Blood Sugar by the Folin-Wu Tube Method
To study the historical Folin-Wu colorimetric method for blood sugar and show how a blank-corrected standard comparison would be calculated. This is practical 1
11. Estimation of SGOT (AST) in Serum
To study the kinetic estimation of serum aspartate aminotransferase, traditionally called SGOT, and calculate the absorbance-change rate from a timed series. Th
12. Estimation of SGPT (ALT) in Serum
To examine a kinetic serum alanine aminotransferase assay, traditionally called SGPT, and calculate a time-normalized absorbance change. This is practical 12 in
13. Estimation of Urea in Serum
To review the historical diacetyl monoxime color method for serum urea and audit its standard and specimen-dilution calculations before reporting a concentratio
14. Estimation of Proteins in Serum by the Biuret Method
To construct a source-based protein standard series for the Biuret color reaction and explain how a serum protein concentration would be read from a verified ca
15. Determination of Serum Bilirubin
To review the measurement of total serum bilirubin using an authenticated diazo method and record the quality checks required before a result is reported. This
16. Determination of Glucose by the Glucose Oxidase Method
To explain a glucose oxidase and peroxidase color assay and calculate a blank-corrected test-to-standard response ratio. This is experiment 16 in the PCI Medici
17. Enzymatic Hydrolysis of Glycogen or Starch by Amylases
To observe the disappearance of an iodine-positive starch reaction during amylase action and relate the observation time to relative hydrolysis under controlled
18. Effect of pH and Temperature on Amylase Activity
To compare amylase activity under different pH and temperature conditions while changing one variable at a time. PCI lists a single experiment on factors affect
19. Preparation of Two Standard Buffer Solutions and Measurement of Their pH
To prepare two instructor-specified buffer solutions and measure each pH with a calibrated meter. PCI lists “any two” standard buffer solutions as practical 19
20. Experiment on Lipid Profile Tests
To identify the measured components of a serum lipid profile and practice one conditional calculation of estimated LDL cholesterol from a complete, verified res
21. Determination of Sodium, Calcium, and Potassium in Serum
To study the measurement and reporting of serum sodium, calcium, and potassium as three distinct analytes in one PCI Medicinal Biochemistry practical. This is i
Theory notes: Medicinal Biochemistry