Pharmaceutical Microbiology Practicals
Year 2 Pharm.D syllabus: PharmacoLearn and PCI.
This subject has fourteen listed practical entries. The articles cover apparatus, sterilisation, staining, motility, enumeration, isolation, identification, antimicrobial testing, sterility tests, MIC, microbial assays, RWC, and diagnostic records. All culture work requires instructor supervision and an organism-specific risk assessment.
Practical exercises
1. Study of Apparatus Used in Experimental Microbiology
To identify the purpose and key safety checks of apparatus used in a teaching microbiology laboratory. This is PCI Pharmaceutical Microbiology practical entry 1
2. Sterilisation of Glassware and Preparation of Media
To document the preparation and verified sterilisation of approved teaching-lab glassware and medium. Follow the college lab manual and instructor's method shee
3. Simple, Gram, and Negative Staining
To compare the three PCI-listed staining methods on instructor-approved teaching slides or low-risk cultures. The assigned organism, reagents, and stain timing
4. Study of Motility Characters
To distinguish true directional microbial motility from Brownian movement using an instructor-approved record or low-risk wet mount. Organism and method are spe
5. Enumeration of Microorganisms: Total and Viable Counts
To distinguish a direct total count from a colony-forming viable count and calculate a teaching example. Sample type, dilution, and counting method are specifie
6. Study of Methods of Pure Culture Isolation
To interpret how an approved isolation method separates a mixed inoculum and to assess a candidate isolated colony. Organisms, media, and incubation are specifi
7. Biochemical Testing for Identification of Microorganisms
To interpret an instructor-supplied biochemical test panel for an approved teaching isolate and document the limits of identification. Isolate, panel, and decis
8. Cultural Sensitivity Testing for Some Microorganisms
To interpret a standardized antimicrobial disk diffusion record from an instructor-approved teaching organism. Record the assigned organism and agents from the
9. Sterility Testing for Powders and Liquids
To interpret a sterility-test record for representative powder and liquid samples under a validated compendial method. Actual sample identity and acceptance dec
10. Determination of Minimum Inhibitory Concentration
To determine the minimum inhibitory concentration, or MIC, from a valid broth-dilution teaching dataset. The organism and drug identities are to be recorded fro
11. Microbiological Assay of Antibiotics by Cup Plate Method
To compare an antibiotic test preparation with a reference standard using a validated cylinder-plate or cup-plate assay record. Antibiotic identity and potency
12. Microbiological Assay of Vitamins by Turbidometric Method
To interpret a vitamin bioassay standard curve and estimate a sample level from a valid turbidimetric record. The vitamin and measured concentration must be ent
13. Determination of RWC
To calculate the syllabus-listed RWC from an instructor-verified disinfectant comparison record. RWC means Rideal-Walker coefficient in this disinfectant-compar
14. Diagnostic Tests for Common Diseases: Widal and Malarial Parasite
To interpret de-identified teaching records for Widal serology and malaria parasite microscopy while recognizing their different diagnostic value. Use a de-iden
Theory notes: Pharmaceutical Microbiology