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Determination of Iodine Value

1. Aim

To determine iodine value from a supervised, validated analytical record. Actual sample and result must be entered in the student record.

2. Requirements

Authenticated oil or fat sample record, validated method sheet, labelled blank and sample titres, standardized reagents, balance record, calculator, protective equipment, and waste instructions. Required inputs: Blank thiosulfate titre B, sample titre S, standardized normality N, and sample mass W. Follow the chosen method sheet for reagent amounts, concentrations, and timings. Any assay acceptance limit is to be recorded from the assigned current monograph or approved college method in the student record against the current approved method or monograph.

3. Theory

Iodine value expresses the mass of iodine absorbed per 100 g of oil or fat under a defined method. It estimates unsaturation and depends on the chosen official procedure. The blank accounts for reagent consumption without sample.

4. Procedure

Identify the authenticated sample, current method, safety controls, and reporting units.
Check the balance, reagent standardization, and blank records before using any titre.
Have the instructor perform or supervise the exact validated analytical procedure; do not substitute an illustrative value for a laboratory setting.
Record original sample and blank readings, including any replicate and validity check.
Apply the formula only to accepted readings and compare the result with the applicable specification, if supplied.

5. Observation and calculation

Student record field	Entry
Case or product	Record here
Method	Record here
Observation	Record here
Inference	Record here
Supervisor check	Record here
Outcome	Record here

Assumed values for calculation practice only: assumed B = 20.0 mL, assumed S = 12.0 mL, assumed N = 0.10, and assumed W = 1.00 g. Assumed iodine value = 12.69 × (20.0 − 12.0) × 0.10 / 1.00 = 10.152 g per 100 g. These invented inputs and output are not a real result, preparation instruction, or acceptance limit. Source formula: Iodine value = 12.69 × (B − S) × N / W, in g iodine per 100 g. Actual measurements must be entered in the student record.

6. Output or final result

Report the value, unit, method, and validity check for the real sample. Actual result for determination of iodine value: Record here.

Teaching-lab safety: Titrants and solvents require instructor supervision, ventilation appropriate to the method, and chemical waste collection. Do not prepare reagents from assumed examples.

This page is a supervised-activity guide; it does not report a completed case.

7. Sources

← Macro, Powder and Microscopic Study of PodophyllumDetermination of Saponification Value and Unsaponifiable Matter →

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