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Demonstration of HPLC
1. Aim
To complete the Year 3 practical demonstration of hplc using a verified method, demonstration, or supplied dataset. The actual sample and finding must be entered in the student record.
2. Requirements
Instructor-approved sample or de-identified instrument record, reference standards, blanks and quality controls, method sheet, calibration record, observation form, calculator or plotting tool, protective equipment, and waste procedure. Exact reagents, volumes, concentrations, settings, and limits are specified in your college lab manual and instructor's method sheet.
3. Theory
High-performance liquid chromatography separates components as mobile phase passes through a packed column. A chromatogram records detector response versus time. Retention time, peak area, resolution, and suitability controls have separate meanings. Instrument conditions and reference injections are essential before assigning peaks.
4. Procedure
- Identify the syllabus entry, sample or dataset, method revision, instrument calibration, and hazards.
- Record reference, blank, control, and sample measurements with units and source IDs.
- Observe the approved method or analyze the supplied dataset without filling missing conditions by guesswork.
- Observe an instructor demonstration or validated instrument video. Trace the solvent path, injector, column, detector, and data system, then annotate a supplied chromatogram.
- Check method suitability, range, and control behaviour before any calculation or interpretation.
- Report raw observations, the justified calculation, limitations, and your college lab manual and instructor's method sheet items separately.
5. Observation and calculation
| Student record field | Entry |
|---|---|
| Case or product | Record here |
| Method | Record here |
| Observation | Record here |
| Inference | Record here |
| Supervisor check | Record here |
| Outcome | Record here |
Assumed values for practice only: An assumed chromatogram has an assumed peak at 4.0 minutes. That invented retention time cannot identify a compound without a matching reference and system suitability. Every invented input and output is assumed, not a real analytical result or operating instruction.
6. Output or final result
Provide a labelled HPLC flow path and chromatogram and conditional interpretation. Record the supervised outcome here.
Teaching-lab safety: Perform chemical or instrument work only under instructor supervision with the approved safety and waste procedures. Assumed values are examples only.
This page is a supervised-activity guide; it does not report a completed case.
7. Sources
- PharmacoLearn Pharm.D Year 3 syllabus
- PCI Pharm.D Regulations and Syllabus
- PharmacoLearn Year 3 Syllabus
- USP Verification of Compendial Procedures
- Method-specific primary reference
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