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Demonstration of GC-MS
1. Aim
To complete the Year 3 practical demonstration of gc-ms using a verified method, demonstration, or supplied dataset. The actual sample and finding must be entered in the student record.
2. Requirements
Instructor-approved sample or de-identified instrument record, reference standards, blanks and quality controls, method sheet, calibration record, observation form, calculator or plotting tool, protective equipment, and waste procedure. Exact reagents, volumes, concentrations, settings, and limits are specified in your college lab manual and instructor's method sheet.
3. Theory
Gas chromatography separates volatile compounds, and mass spectrometry records ion signals that can support identification. A peak retention time and a mass spectrum must be interpreted together with standards, blanks, and library-match limitations. A library hit alone is not confirmation.
4. Procedure
- Identify the syllabus entry, sample or dataset, method revision, instrument calibration, and hazards.
- Record reference, blank, control, and sample measurements with units and source IDs.
- Observe the approved method or analyze the supplied dataset without filling missing conditions by guesswork.
- Observe an approved schematic or instrument demonstration. Label inlet, column, ion source, analyzer, and detector, then inspect a supplied chromatogram and associated spectrum.
- Check method suitability, range, and control behaviour before any calculation or interpretation.
- Report raw observations, the justified calculation, limitations, and your college lab manual and instructor's method sheet items separately.
5. Observation and calculation
| Student record field | Entry |
|---|---|
| Case or product | Record here |
| Method | Record here |
| Observation | Record here |
| Inference | Record here |
| Supervisor check | Record here |
| Outcome | Record here |
Assumed values for practice only: An assumed peak elutes at 6.0 minutes with an assumed dominant ion at m/z 100. These invented signals are not a compound identification. Every invented input and output is assumed, not a real analytical result or operating instruction.
6. Output or final result
Provide a labelled GC-MS instrument chain and conditional interpretation. Record the supervised outcome here.
Teaching-lab safety: Perform chemical or instrument work only under instructor supervision with the approved safety and waste procedures. Assumed values are examples only.
This page is a supervised-activity guide; it does not report a completed case.
7. Sources
- PharmacoLearn Pharm.D Year 3 syllabus
- PCI Pharm.D Regulations and Syllabus
- PharmacoLearn Year 3 Syllabus
- USP Verification of Compendial Procedures
- Method-specific primary reference
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